# Equipment: Lonza 4D-Nucleofector® System

Notice b2dfa2e3489d48b7aabdc1e014bdf508 (solicitation 75N98027R00019) from HEALTH AND HUMAN SERVICES, DEPARTMENT OF, NATIONAL INSTITUTES OF HEALTH NICHD, posted 2026-11-12, responses due 2026-11-18T16:00:00.000Z. Status: open.

| Field | Value |
| --- | --- |
| Notice ID | b2dfa2e3489d48b7aabdc1e014bdf508 |
| Solicitation number | 75N98027R00019 |
| Type | Sources Sought |
| Agency | HEALTH AND HUMAN SERVICES, DEPARTMENT OF |
| Sub-tier | NATIONAL INSTITUTES OF HEALTH |
| Office | NATIONAL INSTITUTES OF HEALTH NICHD |
| Posted | 2026-11-12T22:22:50.000Z |
| Response deadline (UTC) | 2026-11-18T16:00:00.000Z |
| Set-aside | - |
| NAICS | 334516 |
| PSC | 6640 |
| Place of performance | Bethesda, MD, USA |
| Award | - |

## Description

1. Disclaimer THIS IS A SOURCES SOUGHT NOTICE ONLY. This notice is issued solely for information and market research planning purposes. It does not constitute a Request for Quotation (RFQ), Request for Proposal (RFP), or Invitation for Bids (IFB), nor does it restrict the Government to a particular acquisition approach. No contract or purchase order will be awarded from this announcement, and the Government will not reimburse respondents for any costs incurred in preparing a response. 2. Background and Purpose The National Institutes of Health (NIH), Office of the Director, Office of Acquisitions,, Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), Section of Cell Cycle Regulation, has a requirement for a Brand-Name Lonza 4D-Nucleofector® System consisting of the following: 4D-Nucleofector Core Unit, Part No. AAF-1003B – Quantity (1) EA 4D-Nucleofector X Unit, Part No. AAF-1003X - Quantity (1) EA 3. One-year warranty extension for each unit. Or a functional domestic equivalent, to support ongoing NIH-funded research involving the genetic manipulation of mammalian cells. Because NICHD's ongoing studies rely on previously validated electroporation pulse-code workflows, experimental protocols, and Nucleocuvette™ consumables established on a prior-generation Lonza Nucleofector platform, the Lonza 4D-Nucleofector® System is the primary known platform capable of ensuring experimental reproducibility and data continuity without requiring costly and time-consuming protocol re-optimization or re-validation. The Office of Acquisitions is conducting market research to identify qualified vendors—including small businesses, authorized distributors, and domestic manufacturers—and specifically seeks market information to determine: 1. Authorized Lonza Sources: The availability of Lonza Walkersville, Inc. or authorized distributors/resellers (including small businesses, HUBZone, 8(a), SDVOSB, WOSB, and Small Disadvantaged Businesses under NAICS 334516) capable of supplying new Lonza equipment with full manufacturer-backed warranties. 2. Buy American Act (FAR Part 25) Compliance: The availability of any domestic end products manufactured in the United States capable of meeting all functional, warranty, training, and legacy workflow-continuity requirements outlined in Section 3 without requiring experimental re-validation. 3. Technical Requirements and Salient Characteristics To meet the Government's minimum requirements, the proposed system must satisfy all functional and technical specifications from the Statement of Work alongside the laboratory's workflow-continuity constraints: Core Control Unit: The system shall include a Lonza 4D-Nucleofector® Core Unit (or a domestic equivalent central control unit) capable of controlling and operating advanced electroporation-based transfection protocols. Small-Volume Transfection Module: The system shall include a Lonza 4D-Nucleofector® X Unit (or a domestic equivalent functional module) capable of processing small-volume samples (specifically ranging from 20 µL to 100 µL) and supporting transfection of primary cells and other difficult-to-transfect cell types. Nucleic Acid & Reagent Delivery: The system shall support delivery of plasmid DNA, RNA, siRNA, CRISPR/Cas9 reagents, and other nucleic acid-based materials into mammalian cells. Optimized Transfection Performance: The system shall provide optimized electroporation programs designed to maximize transfection efficiency while maintaining high post-transfection cell viability. Sensitive Cell Applications: The system shall support research applications involving primary cells, stem cells, immune cells, and other sensitive cell populations that are not efficiently transfected using conventional lipid-based methods. Software and Protocols: The system shall include manufacturer-provided software and protocols necessary for operation of the instrument. Warranty Coverage: The system shall include a minimum one-year manufacturer warranty and one-year warranty extension coverage for both the Core Unit and X Unit (two years total coverage). Warranty coverage shall begin upon Government acceptance of the equipment and continue in accordance with the manufacturer's warranty terms. Consumable & Legacy Protocol Continuity: If proposing Lonza equipment, the system shall be 100% compatible with commercially available Lonza Nucleofector® reagents, Nucleocuvette™ consumables, and existing pulse-code workflows previously developed and validated on a prior-generation Lonza Nucleofector platform. If proposing a domestic alternative, the vendor must demonstrate that its system and consumables achieve equivalent transfection efficiency and post-transfection cell viability across NICHD's existing mammalian cell workflows without requiring protocol re-optimization or experimental re-validation. Delivery, Installation, and Training: Equipment must be delivered to NIH Bethesda (Building 49) and installed, with on-site or virtual user training for up to five (5) NIH personnel completed within 30 days of installation (covering system operation, software functionality, routine maintenance, and basic troubleshooting). All delivery, installation, and training shall be completed within the 12-month period of performance from award. 4. Capability Statement Submission Instructions Interested organizations that believe they can meet all requirements outlined in Section 3 are invited to submit a tailored Capability Statement (no more than 5 pages) containing the following information: 1. Company Profile: Organization name, mailing address, primary Point of Contact (name, title, email, and telephone number), SAM.gov Unique Entity ID (UEI), and CAGE code. 2. Business Size & Socioeconomic Status: Business size under NAICS 334516 (1,000-employee size standard) and applicable socioeconomic designations (e.g., Small Business, Woman-Owned, Veteran-Owned, Service-Disabled Veteran-Owned, HUBZone, 8(a)). 3. Contract 

## Sources

- View on SAM.gov: https://sam.gov/opp/b2dfa2e3489d48b7aabdc1e014bdf508/view
- Data as of 2026-11-13. Source: https://sam.gov/data-services/Contract%20Opportunities/datagov

## Cite this

"Equipment: Lonza 4D-Nucleofector® System, SAM.gov contract opportunity b2dfa2e3489d48b7aabdc1e014bdf508." samgovapi.io, https://samgovapi.io/opportunity/b2dfa2e3489d48b7aabdc1e014bdf508, data as of 2026-11-13. Primary record: https://sam.gov/opp/b2dfa2e3489d48b7aabdc1e014bdf508/view

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